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Test ID: MBL    
Mannose-Binding Lectin, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Oligomers of mannose-binding lectin (MBL) (functional MBL) are measured by enzyme immunosorbent assay (ELISA) in microtiter plates adsorbed with HYB 131-01 mouse monoclonal antibody. This antibody binds structurally normal MBL from A/A genotype donors, but detects little or no structurally abnormal MBL from other haplotypes. Monoclonal anti-MBL is used both as a capture antibody and as a detection protein in this microtiter immunoassay. The assay is conducted by incubating diluted serum in the microtiter plate, followed by washing and a second incubation with biotin-labeled antibody. After a second wash step, the plate is incubated with streptavidin-horseradish peroxidase (HRP) followed by 3,3',5,5' tetramethylbenzidine (TMB) substrate. Absorbance is read at 450 nm and the results compared to an 8-point standard curve (0, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0, and 40.0 ng/mL).(Package insert: MBL-Oligomer ELISA Kit, BioPorto Diagnostics, Gentofte, Denmark)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Tuesday; 9 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

8 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester