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The presence of IgM class antibody to rubeola is determined by an immunofluorescence assay (IFA). After removal of IgG by specific immunoglobulin antibody, the serum is reacted with rubeola-infected substrate cells on a slide. Specific fluorescence indicating the presence of IgM antibodies to rubeola in the specimen can be detected by an immunofluorescence microscope.(Package insert: Measles Virus Antigen Substrate Slide, BION Enterprises, Des Plaines IL)
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