Test ID: SPUT
Bacterial Culture, Aerobic, Respiratory
Method Description
Describes how the test is performed and provides a method-specific reference
All specimens are screened microscopically by Gram stain to avoid culturing specimens that do not represent lower respiratory secretions; specimens with >25 squamous epithelial cells per low-power field will not be cultured.
Lower respiratory specimens are inoculated onto sheep blood agar, eosin methylene blue agar, and chocolate agar and are incubated for 18 to 24 hours. The following organisms are identified and reported: Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus species, Staphylococcus aureus, Moraxella catarrhalis, Neisseria meningitidis, gram-negative bacilli, and predominant yeast. Other organisms are classified as usual oropharyngeal flora.(Isenberg HD: Clinical Microbiology Procedures Handbook. Edited by HD Isenberg, Washington, DC, ASM Press, 1992, Section 1.15)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously


