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Test ID: CKEL
Creatine Kinase (CK) Isoenzyme Electrophoresis, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Electrophoresis is the migration of charge particles in an electrical field. The rate of migration is dependent on such factors as:

-Net electrical charge of the molecule

-Size and shape of the molecule

-Strength of the electrical field

-Properties of the supporting medium

-Temperature of the operation

 

In this procedure, serum creatine kinase (CK) is separated into 3 isoenzymes by electrophoretic separation on agarose gel.

 

After electrophoresis, the CK isoenzymes are visualized by using a substrate according to the following reactions below:

 

                                                         CK

Creatine phosphate + ADP <-----------> Creatine + ATP

 

 

                                Hexokinase

ATP + D-glucose <-----------> Glucose-6-Phosphate + ADP

 

 

                                G-6-PDH

G-6-P + NADP <------------> NADPH + 6-phosphogluconolactone

 

 

NADPH + PMS <----------> NADP + PMSH

 

 

PMSH + TNBT <-----------> PMS + TNBT reduced (precipitate of formazan)

 

The amount of resulting formazan precipitate is proportional to the CK enzymatic activity.

 

Abbreviations:  

CK:  Creatine Kinase

HK: Hexokinase

G-6-PDH: Glucose-6-Phophate dehydrogenase

ADP: Adenosine 5’Diphosphate

ATP: Adenosine 5’Triphosphate

NADP: Nicotinamide Adenine Dinucleotide Phosphate

NADPH: Nicotinamide Adenine Dinucleotide Phosphate reduced

PMS: Phenazine Methosulfate

PMSH: Phenazine Methosulfate reduced

TNBT: Tetranitro Blue Tetrazolium

 

The films are then dried and densitometry is used to quantitate the bands. (Package insert: Sebia Hydragel ISO-CK, Sebia, Norcross, GA)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

CK, total: Monday through Sunday; Continuously

CK isoenzymes: Monday through Saturday; 12 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day-CK Total 3 day- CK Isoenzymes

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 day-CK Total 4 day- CK Isoenzymes

Performing Laboratory Location The location of the laboratory that performs the test

Rochester