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Unit Code 80619:
Organic Acids Screen, Urine

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Method Description

A urine volume corresponding to 0.25 mg of creatinine is acidified,

then extracted with ethyl acetate.  After separation and evaporation

of the solvent phase, the dry residue is sialylated and analyzed by

capillary GC-MS. When indicated, oximation of 2-keto acids is

performed by reaction with hydroxylamine hydrate.  (Sweetman L:  

Organic acid analysis.  In Techniques in Diagnostic Human

Biochemical Genetics.  Edited by FA Hommes.  Wiley-Liss, New

York, 1991, pp 143-176; ; Hoffman GF, Feyh P:  Organic acid

analysis.  In Physician's Guide to the Laboratory Diagnosis of

Metabolic Diseases.  Edited by N Blau, M Duran, ME Blaskovics

KM Gibson. Berlin, NY, Springer, 2003, pp 27-44)

Performing Laboratory Location

Rochester

Key