Cutaneous Immunofluorescence Antibodies (IgG), Serum
Method Description Describes how the test is performed and provides a method-specific reference
Frozen sections of rhesus monkey esophagus and sodium chloride-split human skin are overlaid with dilutions of patient's serum, incubated, covered with fluorescein-conjugated IgG antiserum, and interpreted with a fluorescence microscope.
Herpes gestationis (Pemphigoid):
An indirect immunofluorescence (IF) test is performed with serum to detect the presence and titer of complement (C3) fixing basement zone (BMZ) antibodies. Cryostat-prepared sections of fresh normal human split-skin substrate are first overlaid with dilutions of patient serum, then covered with normal human serum as a source of complement, and finally with fluorescein-conjugated goat anti-human C3. Serum from patients with Herpes gestationis (pemphigoid) contains IgG BMZ antibodies which are capable of fixing complement, termed "HG factor" or "HG IgG". These antibodies are not detected in other dermatosis of pregnancy.
An indirect IF test is performed with serum to detect the presence and titer of IgG antibodies. Cryostat-prepared sections of fresh rodent bladder substrate are first overlaid with dilutions of patient serum and then covered with fluorescein-conjugated goat anti-human IgG. Serum from patients with paraneoplastic pemphigus contains IgG cell-surface antibodies that bind to simple, columnar and transitional epithelia; BMZ antibodies may also be present. (Immunopathology of the Skin. Edited by EH Beutner, TP Chorzelski, V Kumar. 3rd edition. New York, Wiley Medical Publication, 1987; Gammon WR, Briggaman RA, Inman AO 3rd, et al: Differentiating anti-lamina lucida and anti-sublamina densa anti-BMZ antibodies by indirect immunofluorescence on 1.0 M sodium chloride-separated skin. J Invest Dermatol 1984;82:139-144)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 7 a.m.-5 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
2 days/7 days-Herpes (pemphigoid) gestationis or paraneoplastic pemphigus
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test