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A rapid Calcofluor white stain is used to detect the cysts of Pneumocystis jiroveci (formerly carinii). Calcofluor white is a nonspecific fluorochrome stain that binds with beta-configuration polysaccharides such as cellulose and chitin. Only the cyst stage of Pneumocystis jiroveci (formerly carinii ) stains by this method. Both the 2 internal structures and cyst wall must be seen before calling the slide positive for Pneumocystis jiroveci.
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