Acanthamoeba/Naegleria species, Corneal Scraping or Spinal Fluid
Method Description Describes how the test is performed and provides a method-specific reference
Direct wet preps of the spinal fluid and trichrome-stained smears of the corneal scrapings (if slides are submitted) are examined microscopically. In addition, the specimens are cultured on nonnutrient agar plates prepared using tap water. These plates are also inoculated with the bacterium, Escherichia coli. Coincubation with bacteria enhances the growth of these amebae, which usually occurs after 2 to 4 days of incubation at ambient temperature. The agar surface is examined microscopically for the presence of amebae. (Ma P, Visvesvara GS, Martinez AJ, et al: Naegleria and Acanthamoeba infections. Rev Infect Dis 1990;12:490-513)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test