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Unit Code 80349:
Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

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Method Description

Typing of bacterial isolates is accomplished by comparing the DNA

RFLP patterns of large DNA fragments separated by PFGE of 2 or

more bacterial isolates. Identical RFLP patterns indicate similarity

of the isolates. The test is performed by isolating the DNA from the

organism, cutting the DNA with a restriction enzyme which recognize

10-20 sites on the genome, and separating the fragments using PFGE.

The size pattern of DNA fragments in the electrophoresis gel of

different isolates is then compared.  (Tenover FC, Arbeit RD,

Goering RV, et al:  Interpreting chromosomal DNA restriction patterns

produced by pulsed-field gel electrophoresis:  criteria for bacterial

strain typing.  J Clin Microbiol 1995;33:2233-2239)

Performing Laboratory Location

Rochester

Key