Cryptosporidium Antigen, Feces
Method Description Describes how the test is performed and provides a method-specific reference
Cryptosporidium antigens present in the fecal supernatant are captured by the antibody-coated microtiter wells. A second antibody is added which binds to the complex. This signal is amplified by the addition of antibody biotin conjugate and streptavidin peroxidase. The resulting blue color development indicates the presence of cryptosporidium antigens in the feces. The reaction is completed with acid which changes the blue to yellow and the test is read on a spectrophotometer. Enzyme-linked immunosorbent assay-positive results on stools received in fixative are confirmed by direct fluorescent assay (DFA) before reporting. Frozen stools cannot be confirmed with DFA testing.(Ungar BL: Enzyme-linked immunoassay for detection of cryptosporidium antigens in fecal specimens. J Clin Microbiol 1990;28:2491-2495; Rosenblatt JE, Sloan LM: Evaluation of an immunoassay for the detection of cryptosporidium in stool specimens. J Clin Microbiol 1993;31:1468-1472)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 1 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test