Search our Test Catalog. Specify what to search (name, test code, titles, everywhere) and how to search (begins with, contains)

Unit Code 80333:
Fibroblast Culture for Genetic Testing

Print Friendly View
Print Page Email Page

Method Description

Products of Conception/Autopsy/Stillbirth/Skin Biopsy

The biopsy specimen is cut into small pieces, treated with

collagenase, and placed in a tissue culture flask with Chang's and

MEM alpha-medium, 20% fetal bovine serum, and antibiotics to

establish a fibroblast culture. The cultures are trypsinized into 1 to 3

T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks.

 

Chorionic Villi

The chorionic villi is thoroughly cleaned using sterile

forceps to remove the remaining maternal decidua and blood

clots. The villi are then treated with trypsin and collagenase. The

cells are grown in Chang and MEM-alpha medium for 5 to 10 days.

(Dewald G: Chromosome Study of Autopsy Tissues. In Current

Methods of Autopsy Practice.  2nd edition. Edited by WJ

Ludwig Philadelphia, PA, WB Saunders Co, 1979, 155-159)

Performing Laboratory Location

Rochester

Key