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Test ID: FBC    
Fibroblast Culture for Genetic Testing

Method Description Describes how the test is performed and provides a method-specific reference

Products of Conception/Autopsy/Stillbirth/Skin Biopsy

The biopsy specimen is cut into small pieces, treated with collagenase, and placed in a tissue culture flask with Chang's and MEM alpha-medium, 20% fetal bovine serum, and antibiotics to establish a fibroblast culture. The cultures are trypsinized into 1 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks.

 

Chorionic Villi

The chorionic villi is thoroughly cleaned using sterile forceps to remove the remaining maternal decidua and blood clots. The villi are then treated with trypsin and collagenase. The cells are grown in Chang and MEM-alpha medium for 5 to 10 days. (Dewald G: Chromosome study of autopsy tissues. In Current Methods of Autopsy Practice. 2nd edition. Edited by WJ Ludwig Philadelphia, PA, WB Saunders Co, 1979, 155-159)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

41 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

42 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until reported

Performing Laboratory Location The location of the laboratory that performs the test

Rochester