Chromosome Analysis, Solid Tumors
Method Description Describes how the test is performed and provides a method-specific reference
The tissue is dissociated using enzymes and/or mechanical means and transferred to culture coverslips and/or culture flasks. The cultures are incubated at 37 degrees C with 5% CO2, 5% O2, and 90% N2 for 1 to 10 days depending on cell growth. For harvesting, the cells are treated with colcemid and hypotonic solution, and fixed with glacial acetic acid and methanol. Metaphase cells are dropped onto microscope slides and are routinely stained by G- or Q-banding. Twenty metaphases are usually examined. However, if a clone is suspected, but not confirmed within 20 metaphases, 30 metaphases will be analyzed. Minimal evidence for the presence of an abnormal clone is defined as 2 or more metaphases with the same structural abnormality or chromosomal gain (trisomy), or 3 or more metaphases lacking the same chromosome. Five to 10 metaphases are captured using a computerized imaging system, and 1 or more representative karyograms from each clone are prepared to document the type of abnormality and to permit systematic interpretation of the anomalies.(De Fusco PA, Frytak S, Dahl RJ, et al: Cytogenetic studies in 11 patients with small cell carcinoma of the lung. Mayo Clin Proc 1989;64:168-176; Dewald GW, Dahl RJ, Spurbeck JL, et al: Chromosomally abnormal clones and nonrandom telomeric translocations in cardiac myxomas. Mayo Clin Proc 1987;62:558-567; Jenkins RB, Hay ID, Herath JF, et al: Frequent occurrence of cytogenetic abnormalities in sporadic nonmedullary thyroid carcinoma. Cancer 1990; 66:1213-1220; Mitelman Database of Chromosome Aberrations in Cancer . Edited by F Mitelman, B Johansson, F Mertens. Available from URL: http://cgap.nci.nih.gov/Chromosomes/Mitelman)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
15 to 50 days, depending on culture growth
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Cell pellet: Indefinitely
Performing Laboratory Location The location of the laboratory that performs the test