Giardia antigens present in the stool supernatant are captured by antibodies coating the wells of a microtiter plate. The bound antigen is â€śsandwichedâ€ť by the addition of a second antibody and the signal is amplified through the use of biotin-strepavidin horseradish peroxidase. Blue color develops with the presence of bound antigen. The reaction is stopped with the addition of acid and read visually or with the aid of a spectrophotometer.(Rosenblatt JE, Sloan LM, Schneider SK: Evaluation of an enzyme-linked immunosorbent assay for the detection of Giardia lamblia in stool specimens. Diagn Microbiol Infect Dis 1993;16:337-341)
Monday through Friday; 1 p.m.
Same day/1 day
Fresh Frozen - discarded same day Preserved specimens - 3 days