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Unit Code 8017:
Oligoclonal Banding, Serum and Spinal Fluid

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Method Description

The OCB assay requires paired CSF and serum samples.

Unconcentrated CSF and diluted serum are electrophoresed by IEF.

The separated Ig are visualized by an IgG immunoblot, and OCBs that

are present in the CSF and not in the serum are reported. The assay

uses reagents from Helena Laboratories. (Keir G, Luxton RW,

Thompson EJ:  Isoelectric focusing of cerebrospinal fluid

immunoglobulins G:  an annotated update. Ann Clin Biochem

1990;27:436-443)

Performing Laboratory Location

Rochester

Key