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Unit Code 80085:
Electrophoresis, Protein, Serum

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Method Description

Serum proteins are separated in an electric field according to their size,

shape, and electric charge. The separation is performed on agarose

gels. The proteins are visualized by staining with acid blue and the

intensity of staining is quantitated by densitometry (Helena Quick Scan 2000).

Multiplying by the serum total protein converts the percentage of protein in

each fraction into serum concentration. (Helena SPIFE 3000 Instruction

Manual; Package insert: Helena SPIFE SPE Vis Gel, 2001;

Kyle RA, Katzmann JA, Lust JA, Dispenzieri A: Clinical indications and

applications of electrophoresis and immunofixation. In Manual of Clinical

Laboratory Immunology. 6th edition. Edited by NR Rose, et al.

 Washington, DC, ASM Press, 2002 pp 71-91)

Performing Laboratory Location

Rochester

Key