Unit Code 80027:
B-Cell Lymphoma, FISH, Tissue
Method Description
Hematopathologists at Mayo will examine the paraffin-embedded
tissue blocks and select the region that contains malignant cells
based on comparison with hematoxylin-and-eosin sections. Nuclei
are extracted from paraffin-embedded tissue using a technique in
which tissue cores are processed with xylene, proteinase K, citric
acid, and pepsin. FISH is used to study individual extracted nuclei.
Fusion of CCND1 and IGH; BCL2 and IGH; and c-MYC and IGH are
detected using probes with a dual fusion FISH strategy. Anomalies
involving BCL6 are detected using break-apart FISH probes. At least
200 interphase nuclei are screened for each probe and results for each
abnormal probe(s) are expressed as percent abnormal nuclei.
Performing Laboratory Location
Rochester
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