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Unit Code 800219:
Jo 1 Antibodies, IgG, Serum

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Method Description

Recombinant Jo 1 antigen is coupled covalently to polystyrene

microspheres, which are impregnated with fluorescent dyes to create

a unique fluorescent signature. Jo 1 antibodies, if present in diluted

serum, bind to the Jo 1 antigen on the microspheres. The

microspheres are washed to remove extraneous serum proteins.

Phycoerythrin (PE)-conjugated antihuman IgG antibody is then

added to detect IgG anti-Jo 1 bound to the microspheres. The

microspheres are washed to remove unbound conjugate, and bound

conjugate is detected by laser photometry. A primary laser reveals

the fluorescent signature of each microsphere to distinguish it from

microspheres that are labeled with other antigens, and a secondary

laser reveals the level of PE fluorescence associated with each

microsphere. Results are calculated by comparing the median

fluorescence response for Jo 1 microspheres to a 4-point

calibration curve. (Package insert:  Bioplex 2200 ANA Screen.

Bio-Rad Laboratories, Hercules, CA)

Performing Laboratory Location

Jacksonville

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