Unit Code 800112:
Helicobacter pylori Antibody, IgA, Serum
Method Description
Partially purified Helicobacter pylori antigens are immobilized on
the wells of a microwell plate. Diluted serum is added to the wells.
IgA antibodies, specific to Helicobacter pylori , if present, bind to
the antigen on the microwells. Excess antibodies are washed
away with buffer. Anti-human IgA enzyme conjugate is added to
the wells. The conjugate binds to the antigen-antibody complex
on the plate. The excess enzyme conjugate is washed away and
color is developed by the addition of an enzyme substrate. The
intensity of the color corresponds directly to the amount of
antibody present. (Package insert: GAP[R]-IgM, BIOAMERICA,
Inc., Newport Beach, CA, 2001)
Performing Laboratory Location
Jacksonville
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