Unit Code 800095:
T4 (Thyroxine), Total Only, Serum
Method Description
The Access Total T4 assay is competitive-binding immunoenzymatic
assay. A sample is added to a reaction vessel with antithyroxine
antibody, thyroxine-alkaline phosphatase conjugate, and para-
magnetic particles coated with goat antimouse capture antibody and
a stripping agent to dissociate all T4 from binding proteins. Thyroxine
in the sample competes with the thyroxine-alkaline phophatase
conjugate for binding sites on a limited amount of specific antithyroxine
antibody. Resulting antigen:antibody complexes bind to the capture
antibody on the solid phase. After incubation in a reaction vessel,
materials bound to the solid phase are held in a magnetic field, while
unbound materials are washed away. The chemiluminescent substrate
Lumi-Phos* 530 is added to the vessel and light generated by the
reaction is measured with a luminometer. The light production is
inversely proportional to the concentration of thyroxine in the sample.
The amount of analyte in the sample is determined from a stored,
multipoint calibration curve. (Package Insert: Beckman Coulter Ireland
Inc, Ireland, 2005)
Performing Laboratory Location
Jacksonville
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