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Unit Code 800093:
T3 (Triiodothyronine), Total, Serum

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Method Description

The instrument used is a Beckman Coulter DXI 800. The Access

Free T3 assay is a 2-step competitive-binding immunoenzymatic

assay. Sample is added to reaction vessel with a stripping agent to

dissociate T3 from the binding proteins. T3 in the sample competes

with the T3 analogue coupled to biotin for anti-T3 alkaline phosphatase

conjugate. Of the resulting antigen: antibody complexes, the T3

analogue:antibody complexes are bound to the streptavidin-coated

solid phase. Separation in a magnetic field and washing removes

the sample T3:antibody complexes and other materials not bound

to the solid phase. The chemiluminescent substrate Lumi-Phos* 530

is added to the vessel and light generated by the reaction is measured

with a luminometer. The light production is inversely proportional to the

concentration of total T3 in the sample. The amount of analyte in the

sample is determined from a stored, multipoint calibration curve.

(Package Insert: Beckman Coulter Ireland Inc, Ireland, 2005)

Performing Laboratory Location

Jacksonville

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