Unit Code 800079:
Uric Acid, Urine
Method Description
Uric acid is oxidized by uricase to form allantoin and hydrogen
peroxide. The hydrogen peroxide reacts with TOOS (N-ethyl-N-
(2- hydroxy-3-sulfopropyl)-3-mehtylaniline) and 4-aminophenazone
in the presence of peroxidase to form a quinoneimine dye. The
intensity of the red color formed is proportional to the uric acid
concentration. Prior to the start of the reaction, the sample is initially
incubated with a reagent mixture containing ascorbate oxidase and
a clearing system. This eliminates any ascorbic acid present in
the sample which could interfere with the peroxidase indicator
reaction (Package insert, Roche Uric Acid reagent, Roche Diagnostic
Corp., Indianapolis IN, 1998)
Performing Laboratory Location
Jacksonville
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