Shiga Toxin Escherichia coli Antigen, Feces
Method Description Describes how the test is performed and provides a method-specific reference
An ELISA microplate assay is performed using broth cultures (18-24 hours) of stool specimens placed in microwells coated with rabbit polyclonal anti-Shiga toxin 1 and anti-Shiga toxin 2. In the processing, horseradish peroxidase enzyme conjugate and substrate tetramethylbenzidine (TMB) are added and the developed yellow color is read with a spectrophotometer. Specimens with indeterminate results are repeat tested after overnight incubation in a broth culture. (O'Brien AD, Holmes RK: Shiga and Shiga-like toxins. Microbiol Rev 1987;51:206-220)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test