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Unit Code 800006:
B-Type Natriuretic Peptide (BNP), Plasma

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Method Description

The instrument used is Beckman Coulter DXI 800. The BNP test is a

2-site immunoenzymatic sandwich assay. A sample is added to a

reaction vessel with mouse monoclonal antihuman BNP antibody-

alkaline phosphatase conjugate and paramagnetic particles coated

with mouse monoclonal antihuman BNP antibody. BNP in human

plasma binds to the immobilized anti-BNP on the solid phase, while

the mouse anti-BNP conjugate reacts specifically with bound BNP.

After incubation in a reaction vessel, separation in magnetic field

and washing remove materials not bound to the solid phase. The

chemiluminescent substrate Lumi-Phos* 530 is added to the reaction

vessel and light generated by the reaction is measured. The light

production is directly proportional to the concentration of BNP in the

sample. The amount of analyte in the sample is determined from a

stored, multi-point calibration curve. (Package Insert: Biosite

Incorporated, France 2003)

Performing Laboratory Location

Jacksonville

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