|Values are valid only on day of printing.|
Both immunodiffusion and compliment fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen is a culture filtrate, histoplasmin. H and M precipitin bands are identified. For the CF test, the antigens are histoplasmin and a yeast form of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD: Fungi. In Laboratory Procedures in Clinical Microbiology. Second edition. Edited by JA Washington II. New York, Springer-Verlag, 1985)
The Omega Blastomyces Total Antibody EIA assay uses microwells coated with purified Blastomyces yeast-phase antigen. Patient specimen is diluted in diluent buffer and incubated in the coated microwell. If present, IgG and/or IgM antibodies will bind to the antigen. The microwells are washed to remove unbound serum components. A secondary antibody, rabbit anti-human IgG and IgM antibody conjugated to horseradish peroxidase, is added to the microwell and incubated. The secondary antibody will bind to the antibody-anitigen complexes. The microwells are washed to remove unbound conjugate. Substrate solution containing urea peroxide and tetramethylbenzidine is added to the microwells causing a color change. After a final incubation period, stop solution is added to the microwells and the color change is quantified by measuring the optical density (OD). Sample OD readings are compared to calibrator cutoff OD readings to determine results.(Package insert: Omega Blastomyces Total Antibody EIA, Immuno-Mycologics, Inc., 2700 Technology Place, Norman, OK)
Monday through Friday; 9:30 a.m.