Histoplasma/Blastomyces Antibody Panel, Spinal Fluid
Method Description Describes how the test is performed and provides a method-specific reference
Both immunodiffusion and compliment fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen is a culture filtrate, histoplasmin. H and M precipitin bands are identified. For the CF test, the antigens are histoplasmin and a yeast form of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD: Fungi. In Laboratory Procedures in Clinical Microbiology. Second edition. Edited by JA Washington II. New York, Springer-Verlag, 1985)
The Omega Blastomyces Total Antibody EIA assay uses microwells coated with purified Blastomyces yeast-phase antigen. Patient specimen is diluted in diluent buffer and incubated in the coated microwell. If present, IgG and/or IgM antibodies will bind to the antigen. The microwells are washed to remove unbound serum components. A secondary antibody, rabbit anti-human IgG and IgM antibody conjugated to horseradish peroxidase, is added to the microwell and incubated. The secondary antibody will bind to the antibody-anitigen complexes. The microwells are washed to remove unbound conjugate. Substrate solution containing urea peroxide and tetramethylbenzidine is added to the microwells causing a color change. After a final incubation period, stop solution is added to the microwells and the color change is quantified by measuring the optical density (OD). Sample OD readings are compared to calibrator cutoff OD readings to determine results.(Package insert: Omega Blastomyces Total Antibody EIA, Immuno-Mycologics, Inc., 2700 Technology Place, Norman, OK)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 9:30 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test