Carbapenemase Detection-Carba NP Test
Method Description Describes how the test is performed and provides a method-specific reference
A pure bacterial isolate is emulsified into cell lysis buffer in 2 tubes, 1 of which contains the base indicator solution (phenol red with zinc salts) alone and the other which contains the base indicator solution plus imipenem (6 mg/mL). The tubes are incubated at 37 degrees C for 2 hours. A positive reaction is indicated by a color change from red to yellow as a result of hydrolysis of the beta-lactam ring of imipenem.(Vasoo S, Cunningham SA, Kohner P, et al: Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 2013; 51:3097-3101)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Performing Laboratory Location The location of the laboratory that performs the test