Glucopsychosine, Blood Spot
Method Description Describes how the test is performed and provides a method-specific reference
Methanol containing internal standard is added to a 1/8-inch disk from a dried blood spot. The mixture is rotated at ambient conditions before the eluate is evaporated with nitrogen, the residue reconstituted and injected onto a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Following separation of the structural isomers glucopsychosine (GPSY) and psychosine by liquid chromatography, their concentrations are measured by MS/MS analysis in the multiple reaction monitoring positive mode to follow the precursor to product species transitions for GPSY (m/z 462.4 to m/z 282.4) and the internal standard N,N-dDimethyl-D-erythro-spingosine (m/z 382.4 to m/z 110.20). The ratio of the extracted peak area of GPSY to internal standard is used to calculate the concentration of GPSY in the sample.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday, Friday; 12 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test