|Values are valid only on day of printing.|
Flow cytometric immunophenotyping of peripheral blood (WBC and RBC) is performed using the following antibodies;
RBC: CD235a, CD59
WBC: CD14, CD15, CD16, CD24, CD33, CD45, and FLAER
This assay evaluates the presence or absence of glycosylphosphatidylinositol (GPI)-linked proteins using monoclonal antibodies directed against CD235, CD33, and CD15 to isolate different cell lineages. GPI-linked proteins that are checked within different lineages include CD14 for monocytes, CDâ€™s 16 and 24 for granulocytes, and CD59 for RBCs. Fluorescent aerolysin, a fluorescently labeled inactive variant of the protein aerolysin binds selectively to GPI anchors and is also evaluated for presence or absence of expression on WBCs. In addition, this test will detect a partial loss of CD59 on RBCs (type II RBCs).
Individuals without paroxysmal nocturnal hemoglobinuria have normal expression of all GPI-linked antigens on peripheral blood and leukocytes and erythrocytes.(Richards SJ, Hill A, Hillman P: Recent advances in the diagnosis, monitoring and management of patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom 2007 Sep;72:291-298)
Specimens are processed Monday through Sunday and reported Monday through Friday.