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Test ID: PLINK    
PNH, PI-Linked Antigen, Blood

Method Description Describes how the test is performed and provides a method-specific reference

Flow cytometric immunophenotyping of peripheral blood (WBC and RBC) is performed using the following antibodies;

 

RBC: CD235a, CD59  

WBC: CD14, CD15, CD16, CD24, CD33, CD45, and FLAER  

                                                                    

This assay evaluates the presence or absence of glycosylphosphatidylinositol (GPI)-linked proteins using monoclonal antibodies directed against CD235, CD33, and CD15 to isolate different cell lineages. GPI-linked proteins that are checked within different lineages include CD14 for monocytes, CD’s 16 and 24 for granulocytes, and CD59 for RBCs. Fluorescent aerolysin, a fluorescently labeled inactive variant of the protein aerolysin binds selectively to GPI anchors and is also evaluated for presence or absence of expression on WBCs. In addition, this test will detect a partial loss of CD59 on RBCs (type II RBCs).

 

Individuals without paroxysmal nocturnal hemoglobinuria have normal expression of all GPI-linked antigens on peripheral blood and leukocytes and erythrocytes.(Richards SJ, Hill A, Hillman P: Recent advances in the diagnosis, monitoring and management of patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom 2007 Sep;72[5]:291-298)

Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information

Yes

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Specimens are processed Monday through Sunday and reported Monday through Friday.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days-any remaining

Performing Laboratory Location The location of the laboratory that performs the test

Rochester