Coccidioides Antibody with Reflex, Spinal Fluid
Method Description Describes how the test is performed and provides a method-specific reference
A proprietary mixture of recombinant and native Coccidioides antigens, including the complement-fixing antigen (CF-Ag) and tube precipitin antigen (TP-Ag) are adsorbed to separate microwells. Diluted patient specimens and controls are incubated in both CF-Ag and TP-Ag microwells. If anti-Coccidioides antibodies are present in patient specimens, the antibodies will become bound to the adsorbed antigens. Nonspecific reactants are removed by washing; peroxidase-conjugated, secondary anti-human antibody is then applied to the microwells. If patient antibodies are bound to the adsorbed antigens, the peroxidase-conjugated secondary antibody will bind to the patient antibodies. Excess peroxidase-conjugated secondary antibody is removed by washing. Substrate solution is then added to the microwells, developing color in the presence of peroxidase-conjugated secondary antibody. After adding stop solution, color change is quantified by measuring optical density (OD). Sample OD readings are compared to calibrator cutoff OD readings to determine results.(Package insert: OMEGA Cocci Ab EIA Test Kit, Immuno-Mycologics, Inc., Norman, OK)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday, Sunday; 9 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
1 day (Additional days for CF/ID results if reflex testing is required.)
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test