Hepatitis E Virus IgM Antibody Confirmation, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Highly purified recombinant hepatitis E virus (HEV) antigens (O2N, O2C, and O3 from genotypes 1 and 3, and O2M from genotype 1) are fixed to nitrocellulose membrane test strips. The test strips are incubated with the diluted patient serum sample, allowing virus-specific human IgM antibodies to bind to the antigens present on the test strips. Unbound human antibodies are then washed away, followed by addition of anti-human IgM antibodies that are conjugated with horseradish peroxidase enzyme for incubation. Unbound conjugate antibodies are then washed away. Specifically bound human IgM antibodies are detected with a staining reaction catalysed by the peroxidase. Whenever an antigen-antibody reaction has occurred on the assay strip, a dark band will appear on the strip at the corresponding reactivity location.(Package insert: recomLine HEV IgG/IgM kit, catalog no. 5072, MIKROGEN GmbH, Foster, Neuried, Germany, rev.1/2009)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test