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Test ID: HIV2L    
HIV-2 Antibody Confirmation, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Recombinant proteins and synthetic peptides from HIV-1 and HIV-2, and a synthetic peptide from HIV-1 group O are coated as discrete lines on a nylon strip with plastic backing. Five HIV-1 antigens are applied: sgp120 and gp41, which detect specific antibodies to HIV-1, and p31, p24, and p17, which may also cross-react with antibodies to HIV-2. HIV-1 group O peptides are present in the HIV-1 sgp120 band. The antigens gp36 and sgp105 are applied to detect antibodies specific to HIV-2. In addition to these HIV antigens, 4 control lines are coated on each strip: antistreptavidine line, + or - cutoff line (human IgG), 1+ positive control line (human IgG) and one strong 3+ positive control line, which is also the sample addition control line (antihuman IgG). The INNO-LIA HIV I/II Score is based on the enzyme immunoassay principle. The test sample is incubated in a test trough together with the multiple antigen-coated test strip. HIV antibodies, if present in the sample, will bind to the individual HIV antigen lines on the strip. Afterwards, a goat-antihuman IgG labeled with alkaline phosphatase is added and will bind to any HIV antigen/antibody complex previously formed. Incubation with enzyme substrate (BCIP/NBT) produces a dark brown color in proportion to the amount of HIV antibody present in the sample. Color development is stopped with sulfuric acid.(Package insert: INNO-LIA HIV I/II Score RUO; 28461 v3. 2013-02-13. Innogenetics N.V., Ghent, Belgium)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday; 3 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

5 Days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

9 Days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester