An enzymatic oxalate oxidase method for measuring oxalate in plasma and urine was adapted for dialysate fluid. The reaction yields hydrogen peroxide, which in the presence of peroxidase reacts with a dye to give a colored end point that is measured at 590 nm using a sensitive Beckman Coulter DU800 spectrophotometer.(Ladwig PM, Liedtke RR, Larson TS, Lieske JC: Sensitive spectrophotometric assay for plasma oxalate. Clin Chem 2005;51(12):2377-2380; Wilson DM, Liedtke RR: Modified enzyme-based colorimetric assay of urinary and plasma oxalate with improved sensitivity and no ascorbate interference: reference values and specimen handling procedures. Clin Chem 1991;37:1229-1235)
Monday through Friday; 10 a.m.
Any exceptions needed for the above assay hours will need prior approval from the Renal Laboratory Director or in their absence the Renal Laboratory Supervisor.