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Test ID: 61207    
IDH1/IDH2 Mutation Analysis by Pyrosequencing, Paraffin

Method Description Describes how the test is performed and provides a method-specific reference

Paraffin-embedded tissue is deparaffinized, lysed, and digested. Genomic DNA is then extracted from the sample using either a phenol-chloroform method or the QIAamp DNA FFPE Tissue kit (Qiagen). The DNA is PCR amplified using primers specific for regions surrounding IDH1 codon 132 and IDH2 codon 172. Controls are run with each specimen to assess possible contamination issues and overall test performance. Samples are pyrosequenced and pyrograms are analyzed for the presence or absence of IDH1 codon 132 and IDH2 codon 172 mutations.(Qiagen DNA FFPE Tissue Handbook, Qiagen PyroMark Gold Q24 reagents Handbook; unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 8 a.m.-5 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

4 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

8 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week/7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester