FOXL2 Mutation (C402G) Analysis by PCR and Pyrosequencing
Method Description Describes how the test is performed and provides a method-specific reference
The paraffin-embedded tissue is deparaffinized, lysed, and digested. Genomic DNA is extracted from patient ovarian specimens using either a phenol-chloroform method or the QIAamp DNA FFPE Tissue kit (Qiagen). FOXL2 gene exon 1 is amplified using specific primers by PCR. Controls are run with each specimen to assess possible contamination issues and overall test performance. The PCR products from patient specimen and negative controls are used for pyrosequencing. The pyrograms are analyzed and the presence or absence of the FOXL2 mutation (402C->G) is determined. The results are interpreted by a working group pathologist.(Qiagen DNA FFPE Tissue Handbook, Qiagen PyroMark Gold Q24 reagents Handbook; unpublished Mayo methods)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 8 a.m.-4:30 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test