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Test ID: FLUAB    
Influenza Virus Type A and Type B, Molecular Detection, PCR

Method Description Describes how the test is performed and provides a method-specific reference

The Prodesse ProFlu+ assay is a real-time, reverse-transcription PCR (rRT-PCR) assay for simultaneous detection of influenza A, influenza B, and respiratory syncytial virus (RSV). This assay is modified from the U.S. Food and Drug Administration-approved version by use of an alternate amplification system (Roche LightCycler 480).

 

Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science) from upper and lower respiratory specimens including nasal, throat, and nasopharyngeal swabs, bronchoalveolar lavage, nasopharyngeal aspirates, and bronchial washings and brushings. Reverse transcription produces cDNA from RNA in the extracted specimen, which is then amplified and detected by use of Taqman probes on the Roche LightCycler 480 instrument. Positive results are detected when a fluorescent signal is generated that crosses the threshold of background fluorescence. (Unpublished Mayo method)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location The location of the laboratory that performs the test

Rochester