|Values are valid only on day of printing.|
(125)I-labeled recombinant human antigen (glutamic acid decarboxylase 65, islet antigen 2, or insulin) is added to the test serum. If antibody is present, it forms a soluble complex with the (125)I-labeled antigen. Subsequent addition of goat antihuman IgG and IgM precipitates the complex. The amount of radioactivity in the precipitate is proportional to the level of antibody in the serum.(Masuda M, Powell M, Chen S, et al: Autoantibodies to IA-2 in insulin-dependent diabetes mellitus. Measurements with a new immunoprecipitation assay. Clin Chim Acta 2000;291:53-66)
Monday through Thursday, Sunday; 10 p.m.
GAD65 antibody: Monday through Thursday, Sunday; 8 a.m.
Insulin antibodies: Monday, Thursday; 10 p.m.
IA-2 antibody: Tuesday, Friday; 10 p.m.