HIV-2 Antibody Screen, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The Bio-Rad GS HIV-2 EIA is manufactured from virus that was isolated from the peripheral blood lymphocytes of a West African man. The virus, which is designated HIV-2, is propagated in a CEM cell line. The infected cell line is cultured and the virus is purified by centrifugation. The viral concentrate is disrupted and inactivated using a chaotropic agent and heat before being absorbed onto wells of a microwell plate. Specimens to be tested are diluted in specimen diluent and added to each well, incubated with the absorbed antigen, and washed. If antibodies to the virus are present, they bind to antigen and are not removed by washing. The conjugate reagent, peroxidase-labeled goat antihuman immunoglobulin, is then added to the wells and will bind to the antibody-antigen complex, if present. Unbound conjugate is removed by a wash step. Next, chromogen buffered substrate reagent is added to the plate and allowed to incubate. A blue or blue-green color develops in proportion to the amount of antibody that has been bound to the antigen-coated plate. The enzyme reaction is stopped by the addition of acid. This will result in a color change to yellow. The optical absorbance of controls and specimens is determined with a spectrophotometer with wavelength set at 450 nm.(Package insert: Human Immunodeficiency Virus Type 2 GS HIV-2 EIA: Bio-Rad Laboratories, Redmond, WA)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday, Wednesday, Friday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
1 day. Approximately 1 week for the reflex confirmation result.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test