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Test ID: FFTAS    
FLT3 ITD and TKD Mutation Assay

Method Description Describes how the test is performed and provides a method-specific reference

Polymerase chain reaction (PCR) amplification for the detection of the FLT3 ITD and FLT3 TKD mutations was performed on DNA isolated from the patient sample. Fluorescently labeled primers amplified the sequences of interest. The TKD PCR product was cut with the EcoRV restriction enzyme. The ITD and the digested TKD products were run on the ABI 3500x1 genetic analyzer and their sizes were determined. The ITD wild type produces a fragment that is approximately 327 +/-1 bp, while the presence of an insertion produces a fragment that is approximately >=330 bp. This assay has an analytical sensitivity of 5 mutation containing cells in 100 total cells. This assay does not detect ITD mutations greater than approximately 420 bp in size.

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

3 - 7 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

5 - 9 days

Performing Laboratory Location The location of the laboratory that performs the test

Laboratory for Personalized Molecular Medicine