Test ID: FFLT3
FLT3 Mutation Testing
Method Description 
Describes how the test is performed and provides a method-specific reference
PCR for both the Internal Tandem Duplication (ITD) and the D835 mutation was performed on DNA isolated from the patient sample using fluorescently labeled primers specific for the areas immediately surrounding the ITD and D835 mutations. The PCR product for the ITD is directly run on an ABI 3500x1 genetic analyzer and size of the product determined. The D835 mutation PCR product is cut with the EcoRV restriction enzyme, run on the ABI 3500x1 genetic analyzer, and the sizes of the digested PCR products are determined. This assay has a analytical sensitivity of 5 FLT3 variant containing cells in 100 total cells.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
3 - 5 days
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
5 - 7 days
Performing Laboratory Location The location of the laboratory that performs the test
Laboratory for Personalized Molecular Medicine
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