The PCA3 assay is performed utilizing the PROGENSA assay kit from Gen-Probe, comprised of two quantitative nucleic acid amplification tests, one for PCA3 RNA, one for PSA RNA. In both, the target RNA molecules are isolated from urine specimens utilizing complementary RNA oligonucleotides and a magnetic separation system to capture the target sequences. A reverse transcriptase is utilized to make DNA copies (template) of the target RNA, and an RNA polymerase is utilized to produce multiple RNA copies (amplicon) of the template (transcription-mediated amplification or TMA). Chemiluminescence-labeled complementary probes are utilized to detect the amounts of amplicon present. PCA3 and PSA mRNA are quantiated by comparison with standard curves generated with every assay. The PCA3 score is calculated as 1000 times the ratio of the concentration of PCA3 RNA to the concentration of PSA RNA ([PCA3 mRNA]/[PSA mRNA] x 1000).