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Test ID: FFTRO    
Trofile DNA Co-Receptor Tropism Assay

Available on the App Store

Method Description Describes how the test is performed and provides a method-specific reference

Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.  Trofile DNA uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism tested.

 

Trofile DNA meets the US standards for technical validation as established by the Clinical Laboratory Improvement Amendments.  Trofile DNA is a single cycle pseudovirion based tropism assay that uses the complete gp160 coding region of HIV-1 to evaluate tropism.  Instead of using HIV-1 RNA isolated from patient plasma, Trofile DNA uses cell associated viral DNA taken from whole blood cells infected with HIV.  HIV-1 envelopes encoded by the viral DNA are tested in a cell-based viral infectivity assay in order to determine which co- receptor the HIV-1 virus population is capable of using: CCR5, CXCR4, or both, known as D/M (dual/mixed).

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

14 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

16-20 days

Performing Laboratory Location The location of the laboratory that performs the test

Monogram Biosciences, Inc