Mycobacterium tuberculosis Complex, Pyrazinamide Resistance by pncA DNA Sequencing
Method Description Describes how the test is performed and provides a method-specific reference
Organisms identified as Mycobacterium tuberculosis complex using the Mycobacterium tuberculosis AccuProbe(GenProbe, San Diego, CA) are lysed using the PrepMan Ultra lysis buffer. Using the pncA primers described by Shenai and colleagues, an approximately 700 bp-PCR product is generated that flanks the entire pncA gene and the upstream promoter region. The PCR product is cleaned and sequenced using the Big Dye terminator v 1.1 Cycle Sequencing reagents(Applied Biosystems). Results are analyzed versus the wild-type pncA sequence using MicroSeq Microbial ID software (v2.0). A custom library of non-wild-type sequences was constructed in MicroSeq. An exact match to the custom nucleotide library is required to report the result.(Shenai S, Rodrigues C, Sadani M, et al: Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing. Indian J Tuberc 2009;56:82-90)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
1 day per week, Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test