Unit Code 5363:
X-Ray Microanalysis
Method Description
The fixed tissues received are rinsed three times in 0.1 m sodium
phosphate buffer and dehydrated in a graded series of ethanol and
propylene oxide. The tissues are subsequently infiltrated and
embedded in Spurr's low viscosity embedding resin. Semi-thin
(1 micron) sections for light microscopy are cut with a Reichert-Jung
Ultramicrotome and stained with toluidine blue. Thin sections are cut
with a Reichert-Jung Ultramicrotome and examined with a Philips
CM12/STEM or JEOL 1200 EX II transmission electron microscope
operated at an appropriate kV. Exposures are made at 1,000 to
500,000 magnification on Kodak 4489 electron microscopic film.
Finished 8 x 10 electron micrograph prints are produced at 2.5
enlargement factor on Kodak paper. (Spurr AR: A low-viscosity
epoxy embedding medium for electron microscopy.
J Ultrastruct Res 26:31-43, 1969)
Performing Laboratory Location
Rochester
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