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Test ID: 5363
X-Ray Microanalysis

Method Description Describes how the test is performed and provides a method-specific reference

The fixed tissues received are rinsed three times in 0.1 m sodium

phosphate buffer and dehydrated in a graded series of ethanol and

propylene oxide.  The tissues are subsequently infiltrated and

embedded in Spurr's low viscosity embedding resin.  Semi-thin

(1 micron) sections for light microscopy are cut with a Reichert-Jung

Ultramicrotome and stained with toluidine blue.  Thin sections are cut

with a Reichert-Jung Ultramicrotome and examined with a Philips

CM12/STEM or JEOL 1200 EX II transmission electron microscope

operated at an appropriate kV.  Exposures are made at 1,000 to

500,000 magnification on Kodak 4489 electron microscopic film. 

Finished 8 x 10 electron micrograph prints are produced at 2.5

enlargement factor on Kodak paper.  (Spurr AR:  A low-viscosity

epoxy embedding medium for electron microscopy. 

J Ultrastruct Res 26:31-43, 1969)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Thursday; Varies a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

11 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester