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Unit Code 500610:
Salicylate, Serum

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Method Description

The EMIT assay is a homogeneous enzyme immunoassay

technique used for the analysis of specific compounds in biological

fluids.  The assay is based on competition for antibody binding

sites between drug in the sample and drug labeled with the enzyme

glucose-6-phosphate dehydrogenase (G6P-DH).  Enzyme activity

decreases upon binding to the antibody, so the drug concentration

in the sample can be measured in terms of enzyme activity.  Active

enzyme converts oxidized nicotinamide adenine dinucleotide

(NAD) to NADH, resulting in an absorbance change that is measured

spectrophotometrically. Endogenous serum G6P-DH does not

interfere, because the coenzyme functions only with the bacterial

enzyme employed in the assay.  (EMIT Salicylic Assay package

insert, Syva Company, Dade Behring Inc., Cupertino, CA, October 2005.)

Performing Laboratory Location

New England

Key