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Unit Code 500127:
Vancomycin, Random, Serum

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Method Description

The EMIT assay is a homogeneous enzyme immunoassay technique

used for the analysis of specific compounds in biological fluids.  The

assay is based on competition for antibody binding sites between drug

in the specimen and drug labeled with the enzyme glucose-6-phosphate

dehydrogenase (G6P-DH). Enzyme activity decreases upon binding to

the antibody, so the drug concentration in the specimen can be

measured in terms of enzyme activity. Active enzyme converts oxidized

nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an

absorbance change that is measured spectrophotometrically.

Endogenous serum G6P-DH does not interfere because the coenzyme

functions only with the bacterial enzyme employed in the assay.

(EMIT Vancomycin Assay package insert, Syva Company, Dade

Behring Inc., Cupertino, CA, October 2005.)

Performing Laboratory Location

Rochester

Key