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Unit Code 500124:
Vancomycin, Trough, Serum

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Method Description

The EMIT assay is a homogeneous enzyme immunoassay technique

used for the analysis of specific compounds in biological fluids.  The

assay is based on competition for antibody binding sites between drug

in the specimen and drug labeled with the enzyme glucose-6-phosphate

dehydrogenase (G6P-DH). Enzyme activity decreases upon binding

to the antibody, so the drug concentration in the specimen can be measured

in terms of enzyme activity. Active enzyme converts oxidized nicotinamide

adenine dinucleotide (NAD) to NADH, resulting in an absorbance change

that is measured spectrophotometrically. Endogenous serum G6P-DH

does not interfere because the coenzyme functions only with the bacterial

enzyme employed in the assay.  (EMIT Vancomycin Assay package

insert, Syva Company, Dade Behring Inc., Cupertino, CA, October 2005.)

Performing Laboratory Location

Rochester

Key