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| Web: | MayoMedicalLaboratories.com |
|---|---|
| Email: | mml@mayo.edu |
| Telephone: | 800.533.1710 |
| International: | 507.266.5700 |
| Values are valid only on day of printing. | |
The fixed tissues received are rinsed three times in 0.1 M sodium
phosphate buffer, post-fixed, and stained in 2% osmium tetroxide.
The tissues are then rinsed 3 times in distilled water, en bloc
stained in 2% aqueous uranyl acetate (60 degrees C) for 30 minutes,
and dehydrated in a graded series of ethanol and propylene oxide.
The tissues are subsequently infiltrated and embedded in Spurr's
low viscosity embedding resin. Semi-thin (1 micron) sections for light
microscopy are cut with a Reichert-Jung Ultramicrotome and stained
with toluidine blue. Thin sections are cut with a Reichert-Jung
Ultramicrotome, post-stained with 0.3% aqueous lead citrate, and
examined with a Philips CM12/STEM or JEOL 1200 EXII transmission
electron microscope operated at an appropriate kV. Exposures are
made at 1,000 - 500,000 magnification on Kodak 4489 electron
microscope film. Finished 8 x 10 electron micrograph prints are
produced at 2.5 enlargement factor on Kodak paper. (Spurr AR:
A low-viscosity epoxy resin embedding medium for electron
microscopy. J Ultrastuc Res 1969;26:31-43; McDowell EM, Trump BF:
Histologic fixatives suitable for diagnostic light and electron
microscopy. Arch Pathol Lab Med 1976;100:405-414)