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Test ID: 4993    
Electron Microscopy

Method Description Describes how the test is performed and provides a method-specific reference

The fixed tissues received are rinsed 3 times in 0.1 M sodium phosphate buffer, post-fixed, and stained in 1% osmium tetroxide. The tissues are then rinsed 3 times in distilled water, en bloc stained in 2% aqueous uranyl acetate (60 degrees C) for 30 minutes, and dehydrated in a graded series of ethanol and acetone. The tissues are subsequently infiltrated and embedded in epoxy resin. Semi-thin (1 micron) sections for light microscopy are cut with an ultramicrotome and stained with toluidine blue. Thin sections are cut with an ultramicrotome, post-stained with 0.3% aqueous lead citrate, and examined with a transmission electron microscope operated at an appropriate kV. Images are digitally captured and stored electronically. (McDowell EM, Trump BF: Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Med 1976;100:405-414)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7-21 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

21 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester