Unit Code 460081:
Uric Acid, Serum
Method Description
Uric acid is oxidized by uricase to form allantoin and hydrogen
peroxide. The hydrogen peroxide reacts with TOOS (N-ethyl-N-
[2-hydroxy-3-sulfopropyl]-3-methylaniline) and 4-aminophenazone
in the presence of peroxidase to form a quinine-diimine dye. The
intensity of the red color formed is proportional to the uric acid
concentration. Prior to the start of the reaction, the sample is initially
incubated with a reagent mixture containing ascorbate oxidase
and a clearing system. This eliminates any ascorbic acid present
in the sample that could interfere with the peroxidase indicator
reaction. (Package insert: Roche Uric Acid reagent. Roche
Diagnostic Corp., Indianapolis, IN, November, 2005)
Performing Laboratory Location
New England
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