Helicobacter pylori Antibody, IgA, Serum
Method Description Describes how the test is performed and provides a method-specific reference
This assay is a qualitative ELISA test to detect the presence of IgA class antibodies to Helicobacter pylori in human serum. Partially purified Helicobacter pylori antigens are immobilized onto wells in a microtiter plate. Diluted patient serum is added to the wells and incubated. IgA class antibodies to Helicobacter pylori, if present, bind to the adhered antigen. Excess IgA antibodies are washed away with buffer and an enzyme conjugated anti-human IgA secondary antibody is added to the wells. During incubation, the conjugate binds to the antigen-antibody complex, if present. Excess conjugate is washed away and color is developed by the addition of an enzyme substrate. The color intensity is read on a spectrophotometer. (Package insert: GAP-IgA, BIOAMERICA, Inc., Newport Beach, CA, 2001)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 9 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test