|Values are valid only on day of printing.|
Smooth muscle antibodies (SMA):
The patient's serum in 1:20 and 1:40 dilutions is added to a tissue substrate of mouse stomach/kidney and incubated. Fluorescein-conjugated antiglobulin is then added. The slides are read with a fluorescence microscope.(Doniach D, Roitt IM, Walker JG, Sherlock S: Tissue antibodies in primary biliary cirrhosis, active chronic [lupoid] hepatitis, cryptogenic cirrhosis, and other liver diseases and their clinical implications. Clin Exp Immunol 1966 July;1:237-262)
Anti-mitochondrial antibodies (AMA):
Enzyme immunosorbent assay with purified M2 antigens. This method detects both IgG and IgM antibodies to M2 antigens.(Package insert: Axis-Shield Diagnostics Limited, United Kingdom, August 2000)
Antinuclear antibodies (ANA2):
The method used to detect antinuclear antibody (ANA) is enzyme-linked immunosorbent assay (ELISA). A HEp-2 lysate supplemented with various purified antigens (double-stranded deoxyribonucleic acid (dsDNA), histone, SS-A (Ro), SS-B (La) Smith, RNP, Scl-70, Jo-1, plus centromere antigen) are coated onto microtiter plate wells. A dilution of patient serum is added to the well and incubated. After washing to remove unbound serum protein, an enzyme conjugated antihuman IgG antibody is added to detect human IgG bound to the microtiter plate well. After incubation and washing to remove unbound conjugate, a substrate to the enzyme is added to the well. After incubation, the enzyme substrate reaction is stopped. The complete assay is measured on a spectrophotometer plate reader. The optical density measured is proportional to the antibody present in the patient serum. Testing is performed on the Triturus instrument by Grifols.(Package insert: ELISA kits, Bio-Rad Laboratories, Hercules CA)
Monday through Friday; 9 a.m. and 4 p.m., Saturday; 12 p.m.