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Test ID: WBANF    
HIV-1/-2 Antibody Confirmation Without Immunofluorescence Assay, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Samples are diluted and applied to the nitrocellulose strip containing specific protein bands derived from purified, inactivated HIV-1 strain lymphadenopathy associated virus grown in the CEM cell line. If specific HIV-1 antibodies are present, they bind to these proteins resolved on the strip. Unbound sample is removed by washing. The phosphatase-labeled conjugate is then added to the strip and allowed to incubate. The conjugate attaches to antibody already bound to viral proteins on the strip. Excess conjugate is removed by washing. Color development reagent is then added to the strip. Reaction sites, where enzyme-labeled antibody is bound, are identified by dark bands. The position and intensity of the bands are compared to reference strips developed using positive control sera. All specimens yielding indeterminate or unreadable results are retested, and results are reported based on the best 2 out of 3 replicate testing.(Package insert: GS HIV-1 Western blot. Bio-Rad Laboratories, Redmond, WA)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Stored serum 7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester